From: Charles Plessy Date: Fri, 13 Oct 2017 00:32:18 +0000 (+0900) Subject: Old X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=8541019c82fd002fb9e8b13baee5bcc14f501d55;p=source.git Old --- diff --git a/biblio/16214800.mdwn b/biblio/16214800.mdwn index bc960cc6..5bfec574 100644 --- a/biblio/16214800.mdwn +++ b/biblio/16214800.mdwn @@ -1,3 +1,10 @@ [[!meta title="PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets."]] -[[!tag emulsion]] +[[!tag emulsion PCR]] + +Nucleic Acids Res. 2005 Oct 6;33(17):e150 doi:10.1093/nar/gni143 + +Kojima T, Takei Y, Ohtsuka M, Kawarasaki Y, Yamane T, Nakano H. + +PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets. + [[!pmid 16214800 desc="The composition of the mixture has been modified so that it stays stable in a PCR of 55 cycles of 10 minutes. Uses Sun Soft N° 818SK."]] diff --git a/tags/emulsion.mdwn b/tags/emulsion.mdwn index 4771a2ca..50b4acef 100644 --- a/tags/emulsion.mdwn +++ b/tags/emulsion.mdwn @@ -8,6 +8,10 @@ How to break the droplets ? - In [[Nakano _et al._, 2005|biblio/16233792]], by centrifugation (oil: silicone; superfactant: TritonX-100 0.1%). - In [[Agresti et al., 2005|biblio/16260754]], by centrifugation in presence of diethyl ether (oil: mineral; superfactant: TritonX-100 0.1% and Span 80 4.5%). - - In [[Dielh _et al._, 2006|biblio/16791214]], by pipetting up and down in presence of high salt (100 mM NaCl), SDS (1%), TritonX-100 (1%) (oil: mineral, superfactant: ABIL WE09). + - High salt: + - In [[Dielh _et al._, 2006|biblio/16791214]], by pipetting up and down in 100 mM NaCl, 1 % SDS, 1 % TritonX-100 and Tris (oil: mineral, superfactant: ABIL WE09). + - In [[Kojima _et al._, 2005|biblio|16214800]], by mixing with 1M NaCl, 5 mM Tris-HCl pH 8.0, 0.5 mM EDTA (oil: mineral; superfactant: Sun Soft No. 818SK). + +Different kinds of superfactants: TritonX-100, Span 80, Tween 80 (many articles), ABIL WE09 ([[Dielh _et al._, 2006|biblio/16791214]] and others), Sun Soft No. 818SK (polyglycerol esters of intersesterified ricinoleic acid [[Kojima _et al._, 2005|biblio|16214800]]). [[!inline pages="tagged(emulsion)" actions="no" feedshow=0]]