From: Charles <charles.plessy@oist.jp>
Date: Fri, 10 Jan 2020 04:43:05 +0000 (+0900)
Subject: Café
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=8434cd5dba369ce38e1e3cb322082f7d434b6338;p=source.git

Café
---

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+[[!meta title="Copolymerization of single-cell nucleic acids into balls of acrylamide gel."]]
+[[!tag single_cell emulsion method]
+
+Li S, Kendall J, Park S, Wang Z, Alexander J, Moffitt A, Ranade N, Danyko C,
+Gegenhuber B, Fischer S, Robinson BD, Lepor H, Tollkuhn J, Gillis J, Brouzes E,
+Krasnitz A, Levy D, Wigler M.
+
+Genome Res. 2019 Nov 14. doi:10.1101/gr.253047.119
+
+Copolymerization of single-cell nucleic acids into balls of acrylamide gel.
+
+[[!pmid 31727682 desc="In a droplet, DNA or RNA from a single cell is annealed to a acrydite primer.  An acrylamide gel is then polymerised before emulsion is broken.  DNA strands are then polymerised.  Barcoding is then done by split-and-pool method."]]