From: Charles Plessy Date: Fri, 22 Sep 2017 05:02:48 +0000 (+0900) Subject: Old X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=83f6824f55f954f34cd5a9ad66f2fc88d050e96a;p=source%2F.git Old --- diff --git a/biblio/11782527.mdwn b/biblio/11782527.mdwn new file mode 100644 index 00000000..46eb34b2 --- /dev/null +++ b/biblio/11782527.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Archaeal dUTPase enhances PCR amplifications with archaeal DNA polymerases by preventing dUTP incorporation."]] +[[!tag enzyme PCR]] + +Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):596-601 doi:10.1073/pnas.012372799 + +Hogrefe HH, Hansen CJ, Scott BR, Nielson KB. + +Archaeal dUTPase enhances PCR amplifications with archaeal DNA polymerases by preventing dUTP incorporation. + +[[!pmid 11782527 desc="dTTP is deaminated to dUTP during PCR, which causes the synthesis of uracil-containing DNA, a potent inhibitor of archeal DNA polymerases."]] diff --git a/biblio/To_Do b/biblio/To_Do index 08f37e34..2c4d4751 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -674,9 +674,6 @@ Single-tube nested PCR using two different annealing temperatures. 8663453 [enzymes] Uracil RNA does not have the same inhibiting effect on PCR. -11782527 [enzymes] -dTTP is deaminated to dUTP during PCR, which causes the synthesis of uracil-containing DNA, a potent inhibitor of archeal DNA polymerases. - 11574151 [neurogenin] Unlike mouse ngn3, zebrafish ngn3 is not expressed in the pancreas (but zebrafish ngn1 is).