From: Charles Plessy Date: Fri, 16 Sep 2022 02:33:29 +0000 (+0900) Subject: Polyamines X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=82faeb67c23c86e13d0f5c117b4b4a7366bdfc89;p=source.git Polyamines --- diff --git a/biblio/25096480.mdwn b/biblio/25096480.mdwn new file mode 100644 index 00000000..8743841d --- /dev/null +++ b/biblio/25096480.mdwn @@ -0,0 +1,10 @@ +[[!meta title="An inverse small molecule screen to design a chemically defined medium supporting long-term growth of Drosophila cell lines."]] +[[!tag cell_culture method screen]] + +Burnette M, Brito-Robinson T, Li J, Zartman J. + +Mol Biosyst. 2014 Oct;10(10):2713-23. doi:10.1039/c4mb00155a + +An inverse small molecule screen to design a chemically defined medium supporting long-term growth of Drosophila cell lines. + +[[!pmid 25096480 desc="“Using this approach we developed an intermediate “ZO Fortified” medium, made up of ZO media supplemented with insulin (5 μg mL−1), trehalose (26.4 mM), l-alanyl-l-glutamine (ala-gln, 12 μM), and l-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (A2P, 0.08 μM), with pH adjusted to 6.75.” 410 compounds were screened. “All of the repeated positive hits were polyamines (spermine, spermidine, and putrescine)”"]] diff --git a/tags/cell_culture.mdwn b/tags/cell_culture.mdwn index 53cadb57..b387b0ce 100644 --- a/tags/cell_culture.mdwn +++ b/tags/cell_culture.mdwn @@ -4,7 +4,7 @@ A few notes that just scratch the surface of a vast field… - [[Echalier and Ohanessian (1969)|biblio/4976834]] reported a culture of _Drosophila_ cells that spontaneously transformed. - [[Schneider's (1972)|biblio/4625067]] report of the establishment of the S2 cell line. - - Polyamines are needed for the long-term passaging of some cell lines ([[Mitsuhashi 1998|biblio/9769143]]). + - Polyamines are needed for the long-term passaging of some cell lines ([[Mitsuhashi 1998|biblio/9769143]], [[Burnette and coll., 2014|biblio/25096480]]). - [[Marteijn and coll. (2003)|biblio/12474249]] optimised a culture medium using a genetic algorithm. This methdod has been used at least once in invertebrates ([[Munroe and coll. (2019)|biblio/30747414]]). - [[Kawamura and coll. (2021)|biblio/33899125]] reported the use of plasmin to establish coral cell lines.