From: Charles Plessy Date: Tue, 17 Oct 2017 05:18:40 +0000 (+0900) Subject: Old X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=7cd33e72483ecfe3a6e95e1fefeeaea19a313b70;p=source.git Old --- diff --git a/biblio/11274352.mdwn b/biblio/11274352.mdwn new file mode 100644 index 00000000..33a39fe2 --- /dev/null +++ b/biblio/11274352.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Directed evolution of polymerase function by compartmentalized self-replication."]] +[[!tag selection polymerase emulsion]] + +Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4552-7 doi:10.1073/pnas.071052198 + +Ghadessy FJ, Ong JL, Holliger P. + +Directed evolution of polymerase function by compartmentalized self-replication. + +[[!pmid 11274352 desc="No beads. A bacterial cel is used as a subcompartment maintaining gene and proteins together. Evidence for leakage of small molecules at high compartment density."]] diff --git a/biblio/To_Do b/biblio/To_Do index 1bfc85cd..e76008f6 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -580,12 +580,6 @@ Only the constructs encoding methylases escape degradation. 10471784 [emulsion] The gene product, fused to streptavidin, is bound to a biotinylated molecule in which it is encoded. -12433997 [emulsion] -Random primers were used to introduce mutations. - -11274352 [emulsion] -No beads. A bacterial cel is used as a subcompartment maintaining gene and proteins together. Evidence for leakage of small molecules at high compartment density. - 16308152 [amplification] Detailed protocol for Terminal Continuation (TC).