From: Charles Date: Fri, 14 Feb 2020 03:50:12 +0000 (+0900) Subject: café X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=79e2ce4575b4452c95f50cd35a2fd06520abf757;p=source%2F.git café --- diff --git a/tags/template_switching.mdwn b/tags/template_switching.mdwn index 3945efb2..a0f28cf8 100644 --- a/tags/template_switching.mdwn +++ b/tags/template_switching.mdwn @@ -2,6 +2,8 @@ (work in progress. For alternative cap enrichement methods see the [[cap]] tag page) +### Methods papers. + - 1996: ”Synthesis of high-quality cDNA from nanograms of total or polyA+ RNA with the CapFinder PCR cDNA library construction kit.” Zhu, Y., A. Chenchik and P.D. Siebert. CLONTECHniques 1 1:12-13. Could not find the PDF. @@ -59,6 +61,9 @@ Originally, the TSOs were all-RNA. Since this is expensive to synthesise, TSOs where only the last 3 bases are RNA became popular. LNA was also tested as a replacement for RNA. + - [[Chenchick and coll., 1998|biblio/Chenchick_1998]] reported (rG)n >> rG > + dGdGdG >> rUrUrU. + - [[Picelli et al (2013)|biblio/24056875]] reported a higher performance for RRL compared to RRR, when preparing Smart-seq2 libraries.