From: Charles Date: Mon, 27 Jan 2020 04:09:37 +0000 (+0900) Subject: SMART X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=7959bfeb981b06c140666fe6a9b38c9ae97b00a9;p=source%2F.git SMART --- diff --git a/biblio/11314272.mdwn b/biblio/11314272.mdwn new file mode 100644 index 00000000..c65659a6 --- /dev/null +++ b/biblio/11314272.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Reverse transcriptase template switching: a SMART approach for full-length cDNA library construction."]] +[[!tag template_switching library method]] + +Biotechniques. 2001 Apr;30(4):892-7. + +Zhu YY, Machleder EM, Chenchik A, Li R, Siebert PD. + +Reverse transcriptase template switching: a SMART approach for full-length cDNA library construction. + +[[!pmid 11314272 desc="SMART: “switching mechanism at the 5′ end of the RNA transcript”. Template-swiching with a rGrGrG oligo containing SfiIB, reverse transcription with an oligo-dT primer containing a SfiIA site. Second-strand cDNA synthesis with low-cycle PCR. Cloning with standard methods."]] diff --git a/tags/template_switching.mdwn b/tags/template_switching.mdwn index 351d4908..24ba14f9 100644 --- a/tags/template_switching.mdwn +++ b/tags/template_switching.mdwn @@ -7,6 +7,12 @@ cDNAs with dA, and add 5′ linkers with T4 DNA ligase and duplex adapters ending with a (T)TTTGGG overhang. + - In SMART (switching mechanism at the 5′ end of the RNA transcript), [[Zhu, + Machleder, Chenchik, Li and Siebert (2001)]] , first-strand cDNAs are + prepared with rGrGrG TSOs containing a SfiIB site and oligo-dT RT primers + containing a SfiIA site. Second-strand synthesis with low-cycle PCR, followed + with standard cloning methods. + - The "_terminal continuation_" method ([[Ginsberg et al., 2002|biblio/12462399]], [[Che et al., 2004|biblio/14647400]]) is essentially a template switching with DNA oligonucleotides ending in `CCC` or `GGG`. `AAA`