From: Charles Date: Mon, 27 Jan 2020 04:44:54 +0000 (+0900) Subject: Café X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=78ea2904d4b5b37608337b3e1ec45334190b3521;p=source%2F.git Café --- diff --git a/biblio/11126119.mdwn b/biblio/11126119.mdwn new file mode 100644 index 00000000..736089f5 --- /dev/null +++ b/biblio/11126119.mdwn @@ -0,0 +1,10 @@ +[[!meta title="5' RACE by tailing a general template-switching oligonucleotide."]] +[[!tag template_switching method library]] + +Shi X, Kaminskyj SG. + +Biotechniques. 2000 Dec;29(6):1192-5 doi:10.2144/00296bm07 + +5' RACE by tailing a general template-switching oligonucleotide. + +[[!pmid 11126119 desc="Generate a collection of TSOs by TdT tailing DNA oligos with GTP. As the lengh of the G tail is variable, it is thought to be more suited to the template switching on cDNAs where the C tail is also variable in length."]] diff --git a/tags/template_switching.mdwn b/tags/template_switching.mdwn index d3bef696..0d388dce 100644 --- a/tags/template_switching.mdwn +++ b/tags/template_switching.mdwn @@ -11,8 +11,12 @@ cDNAs with dA, and add 5′ linkers with T4 DNA ligase and duplex adapters ending with a (T)TTTGGG overhang. + - Noticing that the length of the dC tail on the first-strand cDNA is varialbe, + [[Shi and Kaminskyj (2000)|biblio/11126119]] prepared collections of TSOs + with variable rG tail length, using TdT. + - In SMART (switching mechanism at the 5′ end of the RNA transcript), [[Zhu, - Machleder, Chenchik, Li and Siebert (2001)]] , first-strand cDNAs are + Machleder, Chenchik, Li and Siebert (2001)|biblio/11314272]] , first-strand cDNAs are prepared with rGrGrG TSOs containing a SfiIB site and oligo-dT RT primers containing a SfiIA site. Second-strand synthesis with low-cycle PCR, followed with standard cloning methods.