From: Charles Plessy Date: Thu, 31 Oct 2013 08:17:06 +0000 (+0900) Subject: Lu il y a très longtemps. X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=7513b8e933fef5893d4e015d050ff1754e70bd51;p=source%2F.git Lu il y a très longtemps. --- diff --git a/biblio/12089562.mdwn b/biblio/12089562.mdwn new file mode 100644 index 00000000..2e378cf5 --- /dev/null +++ b/biblio/12089562.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Amine-modified random primers to label probes for DNA microarrays."]] +[[!tag microarray amplification method]] + +Xiang CC, Kozhich OA, Chen M, Inman JM, Phan QN, Chen Y, Brownstein MJ. + +Nat Biotechnol. 2002 Jul;20(7):738-42. + +Amine-modified random primers to label probes for DNA microarrays. + +[[!pmid desc="Incorporates aminoallyl-dUTP instead of cy3/5dUTP. Uses priming hexamers with a amino C6dT in 5′. This allows to reduce by 10 folds the quantity of RNA as a starting material. Presence of amplification products of t/rRNAs is not a problem when « optimal » quantities of total RNA are used."]] diff --git a/biblio/To_Do b/biblio/To_Do index e8babc7b..72c86a44 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -22,12 +22,6 @@ Adds a second round of amplification 11687821 Review citing the 2 upper articles -12089562 [amplification] -* Incorporates aminoallyl-dUTP instead of cy3/5dUTP -* uses priming hexamers with a amino C6dT in 5' -This allows to reduce by 10 folds the quantity of RNA as a starting material. -* Presence of amplification products of t/rRNAs is not a problem when «optimal» quantities of total RNA are used. - 12172558 * Says that exponential (65+25 cycles) is more faithful than linear. * Limits [dNTP] to produce only 3' cDNA that are a few hundrer base pairs long.