From: Charles <charles.plessy@oist.jp>
Date: Fri, 8 Mar 2019 04:39:00 +0000 (+0900)
Subject: Document CAPture.
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=735fb4f24844a519806ce282e1ce8e5824afbd5f;p=source%2F.git

Document CAPture.
---

diff --git a/tags/cap.mdwn b/tags/cap.mdwn
index b5bfe73d..6e01b644 100644
--- a/tags/cap.mdwn
+++ b/tags/cap.mdwn
@@ -1,6 +1,10 @@
 [[!meta title="pages tagged cap"]]
 
-Methods for enriching capped RNAs.
+RNA caps
+========
+
+Methods for enriching capped RNAs
+---------------------------------
 
 (work in progress)
 
@@ -14,6 +18,12 @@ Methods for enriching capped RNAs.
 
 (wip: Fromont-racine et al.)
 
+ - CAPture ([[Edery, Chu, Sonenberg and Pelletier, 1995|biblio/7760832]]):
+   a protein fusion of mouse eIF4E (for cap binding) and protein A
+   (for IgG binding) is used to capture capped RNA/cDNA duplexes on a
+   sepharose IgG column.  Completion of first-strand synthesis is ascertained
+   by RNAse A digestion.
+
  - CapSelect ([[Schmidt et al., 1999|biblio/10518626]]): Add a poly-A tail to
    the first-strand cDNA, ligate a double-stranded adapter ending with a
    single-strand (T)TTTGGG overhang, and prime second-strand synthesis.
@@ -30,7 +40,8 @@ Methods for enriching capped RNAs.
  - [[Kwak et al., 2013|biblio/23430654]] modified oligo-capping to create Pro-cap, a method for nuclear
    run-on analysis at single-nucleotide resulution.
 
-Atypical caps (work in progress).
+Atypical caps (work in progress)
+--------------------------------
 
  - 5′-phospho-ADP-ribosylated RNA/DNA cap, synthethysed by RNA
    2′-phosphotransferase Tpt1 ([[Munir, Banerjee and Shuman, 2018|biblio/30202863]]).