From: Charles Plessy <plessy@riken.jp>
Date: Fri, 20 Apr 2018 02:40:19 +0000 (+0900)
Subject: mraw
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=72e4eeb9989bbeae68279fbdc498d26a03978ac2;p=source%2F.git

mraw
---

diff --git a/tags/template_switching.mdwn b/tags/template_switching.mdwn
index e10cf4a5..e7f8577a 100644
--- a/tags/template_switching.mdwn
+++ b/tags/template_switching.mdwn
@@ -21,6 +21,12 @@
    can extend a linker with the sequence of a small RNA via a template switching
    reaction.  (That is: a sRNA can play the same role as a TS oligonucleotide.)
 
+ - in _Capture and Amplification by Tailing and Switching_ (CATS,
+   [[Turchinovich et al (2014)|biblio/24922482]]), short and long RNAs are A-tailed,
+   oligo-dT-primed, and template swiched.  A PNK treatement is needed on
+   circulating RNAs, to remove phosphates or cyclophosphates that would
+   prevent the A-tailing.
+
 ### Effect of chemical composition of the TS oligonucleotide
 
 Originally, the TSOs were all-RNA.  Since this is expensive to synthesise,
@@ -38,6 +44,9 @@ as a replacement for RNA.
  - [[Arguel et al (2017)|biblio/27940562]] reported similar performance for
    RRR and RRL, using a 5′-focused method similar to nanoCAGE or STRT.
 
+ - 3′ phosphate or biotin blocking groups abolish template-switching
+   ([[Turchinovich et al (2014)|biblio/24922482]] and others).
+
 ### Effect of TSO concentration
 
  - For the STRT method, [[Zajac et al (2013)|biblio/24392002]] concluded that