From: Charles Plessy Date: Mon, 16 Oct 2017 04:50:27 +0000 (+0900) Subject: Old X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=713e009246b3aadb44dc11f2510eb236f8077613;p=source.git Old --- diff --git a/biblio/15156154.mdwn b/biblio/15156154.mdwn new file mode 100644 index 00000000..a8c1d037 --- /dev/null +++ b/biblio/15156154.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Generic expansion of the substrate spectrum of a DNA polymerase by directed evolution."]] +[[!tag selection polymerase]] + +Nat Biotechnol. 2004 Jun;22(6):755-9 doi:10.1038/nbt974 + +Ghadessy FJ, Ramsay N, Boudsocq F, Loakes D, Brown A, Iwai S, Vaisman A, Woodgate R, Holliger P. + +Generic expansion of the substrate spectrum of a DNA polymerase by directed evolution. + +[[!pmid 15156154 desc="Polymerases selected on the ability to amplify their gene despite a 3' mismach in the primers were shown to be able to incorporate a broad range of nucleotide analogs."]] diff --git a/biblio/To_Do b/biblio/To_Do index 5e926ce8..2ec868bd 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -604,9 +604,6 @@ Oil droplets containing water droplets can be FACS sorted. 14500846 [emulsion] Improvement of STABLE by addtion of one biotinylated end, usage of wheat germ extract for translation, and reduction of the GC content of the streptavidin gene to facilitate PCR. -15156154 [emulsion] -Polymerases selected on the ability to amplify their gene despite a 3' mismach in the primers were shown to be able to incorporate a broad range of nucleotide analogs. - 16308152 [amplification] Detailed protocol for Terminal Continuation (TC).