From: Charles Plessy Date: Mon, 21 Dec 2015 01:28:49 +0000 (+0900) Subject: Lu il y a longtemps. X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=70e0d58474187eb0a27980624052d4590ddec90c;p=source.git Lu il y a longtemps. --- diff --git a/biblio/26345506.mdwn b/biblio/26345506.mdwn new file mode 100644 index 00000000..00a03a93 --- /dev/null +++ b/biblio/26345506.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Application of an RNA amplification method for reliable single-cell transcriptome analysis."]] +[[!tag single_cell method purification]] + +Biotechniques. 2015 Sep 1;59(3):137-48. doi:10.2144/000114331 + +Suslov O, Silver DJ, Siebzehnrubl FA, Orjalo A, Ptitsyn A, Steindler DA. + +Application of an RNA amplification method for reliable single-cell transcriptome analysis. + +[[!pmid 26345506 desc="Purification by doing nothing: “We observed a substantial loss of template after the PCI procedure; however, we did notice that the inhibition was greatly diminished when RT tubes were simply stored at 40°C for 2–3 days without any cDNA purification. Therefore, we utilized this approach to reduce PCR inhibition.”"]]