From: Charles Plessy Date: Thu, 1 Feb 2018 07:24:36 +0000 (+0900) Subject: Pro-cap X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=6fba0419208d7685c61c54a17fb1b2e5f68a0412;p=source%2F.git Pro-cap --- diff --git a/tags/cap.mdwn b/tags/cap.mdwn index 7442037c..082ef11b 100644 --- a/tags/cap.mdwn +++ b/tags/cap.mdwn @@ -4,6 +4,8 @@ Methods for enriching capped RNAs. (work in progress) + - See the Wikipedia for [[CAGE methods|https://en.wikipedia.org/wiki/Cap_analysis_gene_expression]] (Cap Analysis Gene Expression). + - A method similar to oligo-capping was reported by [[Sekine and Kato|biblio/8247743]] in 1993. - Oligo-capping ([[Maruyama et al., 1994|biblio/8125298]]): dephosphorylate, @@ -21,8 +23,12 @@ Methods for enriching capped RNAs. reads through the cap structure and chemical bond, and integrates the reverse complement of the oligonucleotide to the first-strand cDNA. - - [[Cleptet et al., 2004|biblio/14704363]] modified oligo-capping, to use T4 + - [[Clepet et al., 2004|biblio/14704363]] modified oligo-capping, to use T4 DNA ligase and a double-stranded adapter with NNNNNN overhang instead of T4 RNA ligase and a single-stranded linker. + - [[Kwak et al., 2013|biblio/23430654]] modified oligo-capping to create Pro-cap, a method for nuclear + run-on analysis at single-nucleotide resulution. + + [[!inline pages="tagged(cap)" limit=0]]