From: Charles Plessy Date: Tue, 13 Dec 2022 07:29:08 +0000 (+0900) Subject: tu tudu tu tudu X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=6d1ec6370d9508fe700da8d4b830c57ab02484ed;p=source.git tu tudu tu tudu --- diff --git a/biblio/10.1101_2020.09.08.286724.mdwn b/biblio/10.1101_2020.09.08.286724.mdwn deleted file mode 100644 index 9cea5b64..00000000 --- a/biblio/10.1101_2020.09.08.286724.mdwn +++ /dev/null @@ -1,10 +0,0 @@ -[[!meta title="Cell culture-based shark karyotyping as a resource for chromosome-scale genome analysis"]] -[[!tag bioRxiv cell_culture karyotype]] - -Yoshinobu Uno, Ryo Nozu, Itsuki Kiyatake, Nobuyuki Higashiguchi, Shuji Sodeyama, Kiyomi Murakumo, Keiichi Sato, Shigehiro Kuraku - -bioRxiv 2020.09.08.286724; doi: https://doi.org/10.1101/2020.09.08.286724 - -Cell culture-based shark karyotyping as a resource for chromosome-scale genome analysis - -[[!doi 10.1101/2020.09.08.286724 desc="Primary cell culture of fibroblasts and lymphocytes. Mitogens were used to stimulate lymphocyte growth. “2n = 102 for the whale shark (Rhincodon typus) and zebra shark (Stegostoma fasciatum), and 2n = 106 for the brownbanded bamboo shark (Chiloscyllium punctatum) and whitespotted bamboo shark (C. plagiosum)”. In comparison with teleost cell culture medium, shark cells needed a higher osmolarity and the medium was supplemented with 333 mM urea, 188 mM NaCl and 54 mM trimethylamine N-oxide."]] diff --git a/biblio/33159152.mdwn b/biblio/33159152.mdwn new file mode 100644 index 00000000..bbb40060 --- /dev/null +++ b/biblio/33159152.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Cell culture-based karyotyping of orectolobiform sharks for chromosome-scale genome analysis."]] +[[!tag cell_culture karyotype]] + +Yoshinobu Uno, Ryo Nozu, Itsuki Kiyatake, Nobuyuki Higashiguchi, Shuji Sodeyama, Kiyomi Murakumo, Keiichi Sato, Shigehiro Kuraku + +Commun Biol. 2020 Nov 6;3(1):652. doi:10.1038/s42003-020-01373-7 + +Cell culture-based karyotyping of orectolobiform sharks for chromosome-scale genome analysis. + +[[!pmid 33159152 desc="Primary cell culture of fibroblasts and lymphocytes. Mitogens were used to stimulate lymphocyte growth. “2n = 102 for the whale shark (Rhincodon typus) and zebra shark (Stegostoma fasciatum), and 2n = 106 for the brownbanded bamboo shark (Chiloscyllium punctatum) and whitespotted bamboo shark (C. plagiosum)”. In comparison with teleost cell culture medium, shark cells needed a higher osmolarity and the medium was supplemented with 333 mM urea, 188 mM NaCl and 54 mM trimethylamine N-oxide."]] diff --git a/tags/cell_culture.mdwn b/tags/cell_culture.mdwn index b387b0ce..f4a0970c 100644 --- a/tags/cell_culture.mdwn +++ b/tags/cell_culture.mdwn @@ -8,5 +8,6 @@ A few notes that just scratch the surface of a vast field… - [[Marteijn and coll. (2003)|biblio/12474249]] optimised a culture medium using a genetic algorithm. This methdod has been used at least once in invertebrates ([[Munroe and coll. (2019)|biblio/30747414]]). - [[Kawamura and coll. (2021)|biblio/33899125]] reported the use of plasmin to establish coral cell lines. + - Shark cells need high osmolarity and a supplement of 333 mM urea, 188 mM NaCl and 54 mM trimethylamine N-oxide ([[Uno and coll., 2020|biblio/33159152]]). [[!inline pages="tagged(cell_culture)" limit=0]]