From: Charles Plessy Date: Mon, 16 Oct 2017 00:40:16 +0000 (+0900) Subject: Expand. X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=6ceb5e9e6cc0224415433099b35f5f69717d8a21;p=source.git Expand. --- diff --git a/biblio/14990785.mdwn b/biblio/14990785.mdwn index 9b987ab9..482deef4 100644 --- a/biblio/14990785.mdwn +++ b/biblio/14990785.mdwn @@ -1,3 +1,10 @@ [[!meta title="A novel emulsion mixture for in vitro compartmentalization of transcription and translation in the rabbit reticulocyte system."]] -[[!tag emulsion product not_read]] -[[!pmid 14990785 desc="Uses ABIL EM90"]] +[[!tag emulsion DTT]] + +Protein Eng Des Sel. 2004 Mar;17(3):201-4 doi:10.1093/protein/gzh025 + +Ghadessy FJ, Holliger P. + +A novel emulsion mixture for in vitro compartmentalization of transcription and translation in the rabbit reticulocyte system. + +[[!pmid 14990785 desc="Primary paper for the use of ABIL EM90. Span/Tween superfactants oxydise the reagents. DTT partly prevents oxydisation but inhibits reactions above 20 mM."]] diff --git a/tags/emulsion.mdwn b/tags/emulsion.mdwn index bb76020d..41f66a5c 100644 --- a/tags/emulsion.mdwn +++ b/tags/emulsion.mdwn @@ -14,6 +14,10 @@ How to break the droplets ? - In [[Kojima _et al._, 2005|biblio/16214800]], by mixing with 1M NaCl, 5 mM Tris-HCl pH 8.0, 0.5 mM EDTA (oil: mineral; superfactant: Sun Soft No. 818SK). - Extraction of beads with hexane:oil 1:1, in [[Levy _et al._, 2005|biblio/16131588]] (oil: mineral, superfactant: 5.5% Span 80, 0.5% Tween 80, 0.1 % TritonX-100). -Different kinds of superfactants: TritonX-100, Span 80, Tween 80 (many articles), ABIL WE09 ([[Dielh _et al._, 2006|biblio/16791214]] and others), Sun Soft No. 818SK (polyglycerol esters of intersesterified ricinoleic acid [[Kojima _et al._, 2005|biblio/16214800]]), DC 5225C Formulation Aid / DC 749 Fluid (Dow Chemical Co.) [[Margulies _et al._, 2005|biblio/16056220]]. +Different kinds of superfactants: + - TritonX-100, Span 80, Tween 80 (many articles), + - ABIL WE09 (polysiloxane–polycetyl–polyethylene glycol copolymer; primary paper: [[Dielh _et al._, 2004|biblio/14990785]]) + - Sun Soft No. 818SK (polyglycerol esters of intersesterified ricinoleic acid; primary paper: [[Kojima _et al._, 2005|biblio/16214800]]) + - DC 5225C Formulation Aid / DC 749 Fluid (Dow Chemical Co.) [[Margulies _et al._, 2005|biblio/16056220]] [[!inline pages="tagged(emulsion)" actions="no" feedshow=0]]