From: Charles Plessy Date: Tue, 7 Nov 2017 07:48:39 +0000 (+0900) Subject: Old X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=6aeb9c68757199aa0aa305a5d0f8b086a1f57e2a;p=source%2F.git Old --- diff --git a/biblio/11222780.mdwn b/biblio/11222780.mdwn new file mode 100644 index 00000000..62d9aa84 --- /dev/null +++ b/biblio/11222780.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Quantitative analysis of mRNA amplification by in vitro transcription."]] +[[!tag ssbp reverse_transcription amplification]] + +Nucleic Acids Res. 2001 Mar 1;29(5):E29 + +Baugh LR, Hill AA, Brown EL, Hunter CP. + +Quantitative analysis of mRNA amplification by in vitro transcription. + +[[!pmid 11222780 desc="T7 pol generates aberrant template-unrelated products. This is fixed by reducing the concentration of oligo dT primers, and of enzymes. Under these conditions, 2 rounds of RNA linear amplification is not generatng too much bias. Small-volume protocol. T4gp32, a single stranded protein, increases RT processivity."]] diff --git a/biblio/To_Do b/biblio/To_Do index 153377a6..e246214c 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -38,9 +38,6 @@ Noise due to RNA extraction is 40 times greater than noise due to replicate hybr 12595569 [amplificaiton] Aminoallyl UTP to labal cRNAs. In contrast to cDNAs, DMSO is required for subsequent cyanine labelling. -11222780 [amplification] -T7 pol generates aberrant template-unrelated products. This is fixed by reducing the concentration of oligo dT primers, and of enzymes. Under these conditions, 2 rounds of RNA linear amplification is not generatng too much bias. Small-volume protocol. T4gp32, a single stranded protein, increases RT processivity. - 12161654 [misc] Fluorescent bar-coded oligos to monitor transcription in vivo.