From: Charles Plessy <plessy@riken.jp>
Date: Tue, 5 Dec 2017 02:00:03 +0000 (+0900)
Subject: Ligation to DNA.
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=69124e6dd9158e1d1a13bf3b624e2eefd05eeb76;p=source%2F.git

Ligation to DNA.
---

diff --git a/biblio/14967495.mdwn b/biblio/14967495.mdwn
index bc7e2e53..2cea2745 100644
--- a/biblio/14967495.mdwn
+++ b/biblio/14967495.mdwn
@@ -7,4 +7,5 @@ Yin S, Kiong Ho C, Miller ES, Shuman S.
 
 Characterization of bacteriophage KVP40 and T4 RNA ligase 2.
 
-[[!pmid 14967495 desc="One more tool in the box?"]]
+[[!pmid 14967495 desc="One more tool in the box? Ligation of RNA to an acceptor
+where the last base is DNA is stronlgy suppressed."]]
diff --git a/tags/ligase.mdwn b/tags/ligase.mdwn
index 2490e339..d455a161 100644
--- a/tags/ligase.mdwn
+++ b/tags/ligase.mdwn
@@ -6,6 +6,11 @@ On T4 RNL2:
 
  - Functional residues analysed by [[Yin et al., 2003|biblio/12611899]].
  - The truncated Rnl2 was published by [[Ho et al., 2004|biblio/14962393]].
+ - Ligation of RNA to a DNA acceptor is very weak ([[Yin et al., 2004|biblio/14967495]].
+ - The RNA acceptor strand participates to its ligation by promoting
+   adenylylation of the donor, and by promoting the formation of the
+   phosphodiester bond.  With DNA, this step is 35 times slower
+   ([[Nandakumar et al., 2005|biblio/15851476]]).
  - The K227Q mutation prevents transfer of the andenylyl residue from the
    linker to RNA ends, thus prevents unwanted ligation products. 
    [[Viollet et al., 2011|biblio/21722378]]