From: Charles Plessy <plessy@riken.jp>
Date: Thu, 17 Jul 2014 09:39:11 +0000 (+0900)
Subject: Read on Tuesday.
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=65de7c3c381bf75f5a946b9fd01f85c47060d199;p=source.git

Read on Tuesday.
---

diff --git a/biblio/25003736.mdwn b/biblio/25003736.mdwn
new file mode 100644
index 00000000..48297d21
--- /dev/null
+++ b/biblio/25003736.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Four methods of preparing mRNA 5' end libraries using the illumina sequencing platform."]]
+[[!tag template-switching method oligo-capping benchmark]]
+
+Machida RJ, Lin YY.
+
+PLoS One. 2014 Jul 8;9(7):e101812. doi: 10.1371/journal.pone.0101812
+
+Four methods of preparing mRNA 5' end libraries using the illumina sequencing platform.
+
+[[!pmid 25003736 desc="Capture of 5′ ends via biotinylated forward PCR primers.  CapSMART: ligation of modified oligonucleotides that block RT on 5′-phoshphorylated RNAs (after kinase treatment).  The signal is not much different from standard SMART."]]