From: Charles Plessy <plessy@riken.jp>
Date: Fri, 29 Sep 2017 03:42:15 +0000 (+0900)
Subject: Old
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=644c28c42ca45619dd054dc5f356b016ff6a1add;p=source.git

Old
---

diff --git a/biblio/3299268.mdwn b/biblio/3299268.mdwn
new file mode 100644
index 00000000..651bdd5f
--- /dev/null
+++ b/biblio/3299268.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Synthesis and reactivity of intermediates formed in the T4 RNA ligase reaction."]]
+[[!tag enzyme ligase adenylylation]]
+
+Nucleic Acids Res. 1987 Jul 10;15(13):5289-303.
+
+Hoffmann PU, McLaughlin LW.
+
+Synthesis and reactivity of intermediates formed in the T4 RNA ligase reaction.
+
+[[!pmid 3299268 desc="Synthesis of adenylated RNA.  ssNpR(A) are good acceptors.  pCpN are good donors."]]
diff --git a/biblio/3978074.mdwn b/biblio/3978074.mdwn
new file mode 100644
index 00000000..b43d5086
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+++ b/biblio/3978074.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Donor activation in the T4 RNA ligase reaction. "]]
+[[!tag enzyme ligase adenylylation]]
+
+McLaughlin LW, Piel N, Graeser E.
+
+Biochemistry. 1985 Jan 15;24(2):267-73.
+
+Donor activation in the T4 RNA ligase reaction.
+
+[[!pmid 3978074 desc="Adenylated RNA is a better substrate for T4 RNA ligase."]]
diff --git a/biblio/To_Do b/biblio/To_Do
index 9fd44454..f7e5668b 100644
--- a/biblio/To_Do
+++ b/biblio/To_Do
@@ -83,12 +83,6 @@ Advocates a systematic round of RNA amplificatin, becaus it reduces interarray v
 T4 RNA ligase reaction efficiency improved by PEG and pre-adenylation.
 direct labelling method for big quantities: RNA fragmentation, then T4 RNA ligation of biotinylated oligos.
 
-3978074 [enzymes]
-Adenylated RNA is a better substrate for T4 RNA ligase.
-
-3299268 [enzymes]
-Synthesis of adenylated RNA
-
 9373199 [libraries]
 Oligo-capping to build 5' ou FL cDNA libraries.
 
@@ -830,9 +824,6 @@ HCC (1 mM) promotes intermolecular ligation. This effect is countered by monoval
 386284 [protocols]
 A low ATP concentration contributes to the attainment of high yields. ATP regenerating systems allow to work at sub-stoechiometric concentrations. Rnase A can be used as a SSBP. Spermine enhances yield.
 
-724498 [enzymes]
-Optimal temperature between 0 and 15 degrees. 15-20 % DMSO enhance the reaction. pCp is easily ligated to the 3' end of tRNAs, but the 5' end of tRNAs is difficult to ligate to pCp if it is engaged in a double helix.
-
 9380524 [protocols]
 Concentrations as high as 1 M or 2.5 M can strongly improve the yield.