From: Charles Plessy Date: Wed, 11 Apr 2018 01:09:14 +0000 (+0900) Subject: nanda runda X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=61fa81568d11e203d929e067a3eadceb457aaae2;p=source%2F.git nanda runda --- diff --git a/biblio/29434199.mdwn b/biblio/29434199.mdwn new file mode 100644 index 00000000..d9412a24 --- /dev/null +++ b/biblio/29434199.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Single-cell full-length total RNA sequencing uncovers dynamics of recursive splicing and enhancer RNAs."]] +[[!tag single_cell method transcriptomei ssbp]] + +Nat Commun. 2018 Feb 12;9(1):619. doi:10.1038/s41467-018-02866-0 + +Hayashi T, Ozaki H, Sasagawa Y, Umeda M, Danno H, Nikaido I. + +Single-cell full-length total RNA sequencing uncovers dynamics of recursive splicing and enhancer RNAs. + +[[!pmid 29434199 desc="First-strand cDNAs are synthesised with a RNAse H minus reverse-transcriptase. DNAse I introduces nicks that prime synthesis of new cDNA molecules. T4gp32 promotes the strand-displacement activity of the reverse-transcriptase. Second-strand cDNAs are synthethised with Klenow fragment (3′ → 5′ exo-) primed with NSRs. In single cells, genomic DNA needs to be removed because of the DNAseI digestion during RT and the low-complexity priming of the second-strand synthesis with NSRs. The resulting DNA molecules are tagmented and sequenced with standard methods. Thus, the method is non-stranded. Despite the use of NSRs, the rRNA rate stays between 20 and 30 %."]]