From: Charles Date: Fri, 8 Mar 2019 04:58:05 +0000 (+0900) Subject: Café X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=598b78b7a6a45207092bc70fc3fb68b57a0b44ec;p=source.git Café --- diff --git a/biblio/12777618.mdwn b/biblio/12777618.mdwn new file mode 100644 index 00000000..24066c38 --- /dev/null +++ b/biblio/12777618.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Purifying mRNAs with a high-affinity eIF4E mutant identifies the short 3' poly(A) end phenotype."]] +[[!tag method cap library]] + +Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7033-8 doi:10.1073/pnas.1232347100 + +Choi YH, Hagedorn CH. + +Purifying mRNAs with a high-affinity eIF4E mutant identifies the short 3' poly(A) end phenotype. + +[[!pmid 12777618 desc="Increases cap specificity with a K119A mutation. This mutant is fused to GST instead of protein A."]] diff --git a/tags/cap.mdwn b/tags/cap.mdwn index e3333e7c..afc78ea9 100644 --- a/tags/cap.mdwn +++ b/tags/cap.mdwn @@ -33,6 +33,9 @@ Methods for enriching capped RNAs reads through the cap structure and chemical bond, and integrates the reverse complement of the oligonucleotide to the first-strand cDNA. + - [[Choi and Hagedorn (2003)|biblio/12777618]] improved the CAPture method by using + a K119A mutant of eIF4E. It is fused to GST instead of protein A. + - [[Clepet et al., 2004|biblio/14704363]] modified oligo-capping, to use T4 DNA ligase and a double-stranded adapter with NNNNNN overhang instead of T4 RNA ligase and a single-stranded linker.