From: Charles Date: Thu, 20 Feb 2020 07:16:16 +0000 (+0900) Subject: Café X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=4eec5ac0f42c8577ecc4d50277af239666e13a21;p=source%2F.git Café --- diff --git a/biblio/1279806.mdwn b/biblio/1279806.mdwn new file mode 100644 index 00000000..6e954f17 --- /dev/null +++ b/biblio/1279806.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Mechanism of DNA strand transfer reactions catalyzed by HIV-1 reverse transcriptase."]] +[[!tag reverse_transcriptase]] + +Science. 1992 Nov 13;258(5085):1112-8 doi:10.1126/science.1279806 + +Peliska JA, Benkovic SJ. + +Mechanism of DNA strand transfer reactions catalyzed by HIV-1 reverse transcriptase. + +[[!pmid 1279806 desc="Addition of 1 extra nucleotide happens at an apparent rate of 0.1 / min. Addition of subsequent bases is visible (Fig 3) but much slower. This addition does not requrie RNAseH activity. A > G > T > C ratio on a non-capped oligonucleotide duplex: 1 : 0.5 : 0.2 : 0.07, determined by incubating the reaction with each individual dNTP. 50 mM Tris-HCl pH 8, 75 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 200 nM DNA/RNA duplex, each dNTP at 100 µM, 7 mM MgCl2, 800 nM TSO and 200 nM HIV-1 RT, at 37 °C."]] diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn index 3e3d5b97..d57c0a65 100644 --- a/tags/reverse_transcription.mdwn +++ b/tags/reverse_transcription.mdwn @@ -36,6 +36,15 @@ _(redaction in progress)_ Like other DNA polymerases ([[Clark, 1988|biblio/2460825]]), reverse transcriptases have a TdT activity. +Peliska JA, Benkovic SJ. + +Mechanism of DNA strand transfer reactions catalyzed by HIV-1 reverse transcriptase. + + - [[Peliska and Benkovic, 1992|biblio/1279806]] showed that HIV-1 RT adds one + nucleotide on non-capped oligonucleotide duplexes. A > G > T > C ratio: + 1 : 0.5 : 0.2 : 0.07. Addition of subsequent bases is visible but much + slower. + - [[Patel & Preston, 1994|biblio/7507249]] showed that HIV RT adds one nucleotide (A > G >> T|C) on DNA/DNA duplexes, and more on DNA/RNA duplexes. Addition is favoured by increased dNTP levels. MMLV and AMV were also