From: admin Date: Mon, 15 Jun 2020 07:55:32 +0000 (+0000) Subject: updated PO files X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=4d5b516d6efdb471cca8b4465be81a06a4a4d7be;p=source.git updated PO files --- diff --git a/open-source-biologist.en.po b/open-source-biologist.en.po index 20b07d75..1cf95c10 100644 --- a/open-source-biologist.en.po +++ b/open-source-biologist.en.po @@ -7,7 +7,7 @@ msgid "" msgstr "" "Project-Id-Version: PACKAGE VERSION\n" -"POT-Creation-Date: 2020-06-15 07:48+0000\n" +"POT-Creation-Date: 2020-06-15 07:55+0000\n" "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n" "Last-Translator: FULL NAME \n" "Language-Team: LANGUAGE \n" @@ -26,27 +26,31 @@ msgid "" "My training as a researcher started with **developmental genetics in " "drosophila and zebrafish**, where I studied the activity of transcription " "enhancers ([Blader and coll., 2003](https://pubmed.gov/12559493)) and their " -"evolutionary conservation (Plessy et al., 2005). This gave me a strong " -"interest for whole-transcriptome analysis and technology. For that purpose, " -"I have joined RIKEN in 2004, where have worked on high-throughput methods " -"for **profiling promoters and inferring gene networks**, and in particular " -"on CAGE (Cap Analysis Gene Expression)." +"evolutionary conservation ([Plessy and coll., 2005](https://pubmed." +"gov/15797614)). This gave me a strong interest for whole-transcriptome " +"analysis and technology. For that purpose, I have joined RIKEN in 2004, " +"where have worked on high-throughput methods for **profiling promoters and " +"inferring gene networks**, and in particular on CAGE (Cap Analysis Gene " +"Expression)." msgstr "" #. type: Plain text msgid "" "I have developed a miniaturized version of CAGE, termed **nanoCAGE**, to " -"analyse small samples yielding only nanograms of RNA (Plessy et al., 2010). " -"In the same manuscript, we also introduced its paired-end variant, " -"**CAGEscan**, which we use to **associate novel promoters with " -"annotations**. Since then, we have kept improving or expanding these " -"techniques, by updating the protocol (Salimullah et al., 2011), reducing the " -"sequence bias introduced by the molecular barcodes (Tang et al., 2013), " -"combining multiple cap-enrichment steps (Batut et al., 2013), benchmarking " -"the use of locked nucleic acids for template switching (Harbers et al., " -"2013), and reducing the number of primer artefacts and unwanted sequences " -"generated by ribosomal RNAs using low-complexity “pseudo-random” reverse-" -"transcription primers (Arnaud et al., 2016)." +"analyse small samples yielding only nanograms of RNA ([Plessy and coll., " +"2010](https://pubmed.gov/20543846)). In the same manuscript, we also " +"introduced its paired-end variant, **CAGEscan**, which we use to **associate " +"novel promoters with annotations**. Since then, we have kept improving or " +"expanding these techniques, by updating the protocol ([Salimullah and coll., " +"2011](https://pubmed.gov/21205859)), reducing the sequence bias introduced " +"by the molecular barcodes ([Tang and coll., 2013](https://pubmed." +"gov/23180801)), combining multiple cap-enrichment steps ([Batut and coll., " +"2013](https://pubmed.gov/22936248)), benchmarking the use of locked nucleic " +"acids for template switching ([Harbers and coll., 2013](https://pubmed." +"gov/24079827)), and reducing the number of primer artefacts and unwanted " +"sequences generated by ribosomal RNAs using low-complexity “pseudo-random” " +"reverse-transcription primers ([Arnaud and coll., 2016](https://pubmed." +"gov/27071605))." msgstr "" #. type: Plain text