From: Charles Date: Mon, 27 Jan 2020 04:33:51 +0000 (+0900) Subject: CapFinder. X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=4c5a1ad90ad8d516ab6425b105466146b670f426;p=source.git CapFinder. --- diff --git a/tags/template_switching.mdwn b/tags/template_switching.mdwn index 24ba14f9..d3bef696 100644 --- a/tags/template_switching.mdwn +++ b/tags/template_switching.mdwn @@ -2,6 +2,10 @@ (work in progress. For alternative cap enrichement methods see the [[cap]] tag page) + - 1996: ”Synthesis of high-quality cDNA from nanograms of total or polyA+ RNA + with the CapFinder PCR cDNA library construction kit.” Zhu, Y., A. Chenchik + and P.D. Siebert. CLONTECHniques 1 1:12-13. Could not find the PDF. + - In the "_CapSelect_" method, [[Schmidt and Mueller, 1999|biblio/10518626]] stimulate template switching with manganese (see below), tail the first-strand cDNAs with dA, and add 5′ linkers with T4 DNA ligase and duplex adapters