From: Charles Plessy Date: Wed, 14 Feb 2018 07:39:40 +0000 (+0900) Subject: Old X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=4b0936c2d43effa0e4a139ac5b924daa07a94915;p=source.git Old --- diff --git a/biblio/20716356.mdwn b/biblio/20716356.mdwn new file mode 100644 index 00000000..f14389d7 --- /dev/null +++ b/biblio/20716356.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Multi-targeted priming for genome-wide gene expression assays."]] +[[!tag random_priming method]] + +BMC Genomics. 2010 Aug 17;11:477. doi:10.1186/1471-2164-11-477 + +Adomas AB, Lopez-Giraldez F, Clark TA, Wang Z, Townsend JP. + +Multi-targeted priming for genome-wide gene expression assays. + +[[!pmid 20716356 desc="NDKTBBBBDWGS occurs one or more times in 76% of 6608 mRNAs present in budding yeast, but is absent in rRNA or tRNA."]] diff --git a/biblio/9973624.mdwn b/biblio/9973624.mdwn index af76a848..a2f3f880 100644 --- a/biblio/9973624.mdwn +++ b/biblio/9973624.mdwn @@ -1,5 +1,5 @@ [[!meta title="Increased specificity of reverse transcription priming by trehalose and oligo-blockers allows high-efficiency window separation of mRNA display."]] -[[!tag thermo-enhancement enzyme random_priming]] +[[!tag thermo-enhancement enzyme random_priming reverse_transcription]] Mizuno Y, Carninci P, Okazaki Y, Tateno M, Kawai J, Amanuma H, Muramatsu M, Hayashizaki Y. diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn index 9d9ac9d8..24fa500a 100644 --- a/tags/reverse_transcription.mdwn +++ b/tags/reverse_transcription.mdwn @@ -15,9 +15,13 @@ Reverse-transcriptases have a DNA-dependent DNA polymerase activity. but not RNA-dependent polymerase activity and is used to suppress these artefacts ([[Perocchi et al., 2007|biblio/17897965]], [[Kanamori-Katayama et al., 2011|biblio/21596820]]). +Reverse-transcriptases tolerate terminal mismatches: [[Mizuno et al.,1999 |biblio/9973624]]. + Reverse-transcription primers: - "N15" random pentadecamers: [[Stangegaard et al., 2006|biblio/16708763]]. - - ... and many more (not-so-random; pseudo-random, ...) + - Multi-targeted primers (MTP): [[Adomas et al., 2010|biblio/20716356]]. + - "Not-so random" (NSR) primers: [[Armour et al., 2009|biblio/19668204]]. + - ... and many more (pseudo-random, ...) [[!inline pages="tagged(reverse_transcription)" actions="no" limit=0]]