From: Charles Date: Mon, 17 Feb 2020 04:47:45 +0000 (+0900) Subject: Café X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=4a67c8baae55ccf36bb341bb61b155274c807c5d;p=source.git Café --- diff --git a/biblio/8534373.mdwn b/biblio/8534373.mdwn new file mode 100644 index 00000000..dcc8c296 --- /dev/null +++ b/biblio/8534373.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Transcription of the 5'-terminal cap nucleotide by RNA-dependent DNA polymerase: possible involvement in retroviral reverse transcription."]] +[[!tag reverse_transcriptase cap]] + +Volloch VZ, Schweitzer B, Rits S. + +DNA Cell Biol. 1995 Dec;14(12):991-6 doi:10.1089/dna.1995.14.991 + +Transcription of the 5'-terminal cap nucleotide by RNA-dependent DNA polymerase: possible involvement in retroviral reverse transcription. + +[[!pmid 8534373 desc="Utilises a 5′ hairpin in the beta-globin mRNA to design a cDNA extension assay where the reverse-transcribed cap creates a terminal mismatch that inhibits extension. De-capping the RNA removes the inhibition. 40 mM Tris 8.3, 40 mM KCl, 5 mM MgCl2, 2 mM DTT. AMV, 45°C"]] diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn index 51efdcd8..3e3d5b97 100644 --- a/tags/reverse_transcription.mdwn +++ b/tags/reverse_transcription.mdwn @@ -79,8 +79,9 @@ nucleotide as a template. at the 5′ end of cDNA clones, and concluded that the cap can be reverse-transcribed. They supported their conclusion with molecular modelling. - - [[Volloch et al, 1995|biblio/8534373]] studied cap transcription (but I could - not access the article). + - [[Volloch, Schweitzer and Rits, 1995|biblio/8534373]] showed that the AMV + reverse-transcribes the cap, using an assay where the extension of a natural + hairpin in the beta-globin cDNA can be enabled by decapping. - [[Kulpa, Topping and Telesnitsky, 1997|biblio/9049314]] noted the addition of one G at the position where the 5′ end of the MMLV RNA was