From: Charles Plessy <charles.plessy@oist.jp>
Date: Fri, 8 Feb 2019 07:32:29 +0000 (+0900)
Subject: RT specificity
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=4981a5c7b552b20f946a3a45f4d4be7cd10baa8f;p=source.git

RT specificity
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+[[!meta title="Detection of splice isoforms and rare intermediates using multiplexed primer extension sequencing."]]
+[[!tag reverse_transcription method]]
+
+Nat Methods. 2019 Jan;16(1):55-58. doi:10.1038/s41592-018-0258-x
+
+Xu H, Fair BJ, Dwyer ZW, Gildea M, Pleiss JA.
+
+Detection of splice isoforms and rare intermediates using multiplexed primer extension sequencing.
+
+[[!pmid 30573814 desc="Specificity of RT priming can be increased by increasing temperature from 47 °C to 55 °C."]]