From: Charles Plessy <plessy@riken.jp>
Date: Wed, 15 Feb 2017 03:48:38 +0000 (+0900)
Subject: Lu dans le lit.
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=483e14e82e2627223587ef8179f2cee8f47e392e;p=source.git

Lu dans le lit.
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+[[!meta title="Short structured RNAs with low GC content are selectively lost during extraction from a small number of cells."]]
+[[!tag purification method Trizol miRNA bias]]
+
+Mol Cell. 2012 Jun 29;46(6):893-5. doi:10.1016/j.molcel.2012.05.036
+
+Kim YK, Yeo J, Kim B, Ha M, Kim VN.
+
+Short structured RNAs with low GC content are selectively lost during extraction from a small number of cells.
+
+[[!pmid 22749402 desc="In extraction methods using Trizol, the long RNAs present in the cell lysate
+act as carrier for the short RNAs, and when initial cell numbers are low or magnesium ions
+are chelated, structured small RNAs become selectively harder to capture, which causes
+a bias that can be confounded with key experimental parameters."]]