From: Charles <charles.plessy@oist.jp>
Date: Tue, 8 Jan 2019 07:39:02 +0000 (+0900)
Subject: Café
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=46bbfcf31dd1fac047a0ca72ae8254ba9008437e;p=source.git

Café
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+[[!meta title="A High-Quality De Novo Genome Assembly from a Single Mosquito using PacBio Sequencing"]]
+[[!tag genome assembly mosquito method extraction]]
+
+Sarah Kingan, Haynes Heaton, Juliana Cudini, Christine Lambert, Primo Baybayan, Brendan Galvin, Richard Durbin, Jonas Korlach, Mara Lawniczak
+
+bioRxiv preprint
+
+A High-Quality De Novo Genome Assembly from a Single Mosquito using PacBio Sequencing
+
+[[!doi 10.1101/499954 desc="Single individual ground with no twisting (only up-down movements).  DNA extracted with Qiagen MagAttract HMW kit, gentle flipping and wide-bore tips.  One contig was a bacterial genome, probably from gut flora.  Two contigs contained multiple copies of the mitochondrial chromosome.  Comparison with Sanger assembly corrected repeat compression."]]