From: Charles Plessy Date: Mon, 14 Nov 2011 11:29:32 +0000 (+0900) Subject: Nouveaux articles et consolidations sur cellules isolées. X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=3fa58ec0a50ceb4968454a3737d68c19199804ed;p=source.git Nouveaux articles et consolidations sur cellules isolées. --- diff --git a/biblio/12202777.mdwn b/biblio/12202777.mdwn new file mode 100644 index 00000000..33c5afda --- /dev/null +++ b/biblio/12202777.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Improved quantitative real-time RT-PCR for expression profiling of individual cells."]] +[[!tag single_cell method PCR inhibition]] + +Liss B. + +Nucleic Acids Res. 2002 Sep 1;30(17):e89. + +Improved quantitative real-time RT-PCR for expression profiling of individual cells. + +[[!pmid 12202777 desc="Single cell precipitation protocol, because reverse transcriptase from the cDNA mixture inhibits PCR."]] diff --git a/biblio/16314311.mdwn b/biblio/16314311.mdwn new file mode 100644 index 00000000..33f7e238 --- /dev/null +++ b/biblio/16314311.mdwn @@ -0,0 +1,10 @@ +[[!meta title="PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency."]] +[[!tag method reverse-transcription PCR inhibition]] + +Suslov O, Steindler DA. + +Nucleic Acids Res. 2005 Nov 27;33(20):e181. + +PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency. + +[[!pmid 16314311 desc="Phenol / Chloroform / Isoamyl alcohol (PCI) treatment followed by ethanol precipitation strongly decreases Ct as well as increases the consistencies of the comparisons between different dillutions of the same samples"]] diff --git a/biblio/16642997.mdwn b/biblio/16642997.mdwn index 60280045..e4f80222 100644 --- a/biblio/16642997.mdwn +++ b/biblio/16642997.mdwn @@ -1,3 +1,3 @@ [[!meta title="Microfluidic single-cell mRNA isolation and analysis."]] -[[!tag microfluidics single-cell]] +[[!tag microfluidics single_cell]] [[!pmid 16642997 desc="The RNA are manipulated with magnetic beads which carry oliognuclotides serving as support and as RT primers."]] diff --git a/biblio/17071717.mdwn b/biblio/17071717.mdwn index 8558a030..06041cf1 100644 --- a/biblio/17071717.mdwn +++ b/biblio/17071717.mdwn @@ -1,3 +1,3 @@ [[!meta title="Gene expression profiling of single cells on large-scale oligonucleotide arrays."]] -[[!tag single-cell amplification oligonucleotides]] +[[!tag single_cell amplification oligonucleotides]] [[!pmid 17071717 desc="High concentration of a dT and random primers mix in reverse transcription."]] diff --git a/biblio/19363473.mdwn b/biblio/19363473.mdwn index 20cd88fe..7813e7f7 100644 --- a/biblio/19363473.mdwn +++ b/biblio/19363473.mdwn @@ -1,3 +1,3 @@ [[!meta title="Non-genetic origins of cell-to-cell variability in TRAIL-induced apoptosis."]] -[[!tag single-cell]] +[[!tag single_cell]] [[!pmid 19363473 desc="“Protein state is transmitted from mother to daughter, giving rise to transient heritability in fate, but protein synthesis promotes rapid divergence so that sister cells soon become no more similar to each other than pairs of cells chosen at random.”"]] diff --git a/biblio/20110323.mdwn b/biblio/20110323.mdwn index edf4c71f..08023949 100644 --- a/biblio/20110323.mdwn +++ b/biblio/20110323.mdwn @@ -1,3 +1,3 @@ [[!meta title="Digital nature of the immediate-early transcriptional response."]] -[[!tag single-cell transcription]] +[[!tag single_cell transcription]] [[!pmid 20110323 desc="Dictyostelium slugs stimulated with cAMP and DIF show a dose-dependent mosaic response."]] diff --git a/biblio/To_Do b/biblio/To_Do index 7ac6c309..f178374c 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -148,9 +148,6 @@ Ligation of cDNA and methylated oligos, in presence of methy-sensitive restricti 14513556 [libraries] Hybridises single-stranded tester plasmid and driver PCR product, then purified single strand molecules out of duplexes using hydroxyapatite. -12202777 [single cells] [tracked] -Single cell precipitation protocol, becausr Reverse Transcriptase from the cDNA mixture inhibits PCR. - 12161758 [amplification] A method to amplify two distinguishable substrates in the same PCR tube, so that they are subjected to the same biases. @@ -822,9 +819,6 @@ Polymerases selected on the ability to amplify their gene despite a 3' mismach i 7760832 [libraries] An eIF-4E fusion protein is used to bind the caps of RNA/DNA duplexes. RNase A cuts single stranded RNAs, thus separating the complexes from the cDNA if they are not full length. -16314311 [enzymes] -Phenol / Chloroform / Isoamyl alcohol (PCI) treatment followed by ethanol precipitation strongly decreases Ct as well as increases the consistencies of the comparisons between different dillutions of the same samples. - 16308152 [amplification] Detailed protocol for Terminal Continuation (TC).