From: Charles Plessy Date: Thu, 24 Aug 2017 00:35:35 +0000 (+0900) Subject: Vieux. X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=3d35e8fc5a13c03883e5c3d6af1ea8b720a08b0b;p=source%2F.git Vieux. --- diff --git a/biblio/11768647.mdwn b/biblio/11768647.mdwn new file mode 100644 index 00000000..c012fdef --- /dev/null +++ b/biblio/11768647.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Serial analysis of gene expression in a single cell."]] +[[!tag sequence_tags single_cell]] + +Schober MS, Min YN, Chen YQ. + +Biotechniques. 2001 Dec;31(6):1240-2. + +Serial analysis of gene expression in a single cell. + +[[!pmid 11768647 desc="Lysis in Dynabeads buffer, followed by first strand synthesis from poly-A."]] diff --git a/biblio/9742245.mdwn b/biblio/9742245.mdwn new file mode 100644 index 00000000..c302e665 --- /dev/null +++ b/biblio/9742245.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Expression profiling of single cells using 3 prime end amplification (TPEA) PCR."]] +[[!tag single_cell]] + +Dixon AK, Richardson PJ, Lee K, Carter NP, Freeman TC. + +Nucleic Acids Res. 1998 Oct 1;26(19):4426-31. + +Expression profiling of single cells using 3 prime end amplification (TPEA) PCR. + +[[!pmid 9742245 desc="Nucleus removed by spinning. 1st PCR with poly-T and random primer. 2nd PCR nested and gene specific."]] diff --git a/biblio/To_Do b/biblio/To_Do index 6698258d..c9e05000 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -153,9 +153,6 @@ Engeneered T7 used to generate dye-terminated amplification products. 15271495 [single cell] Review -9742245 [single cell] [tracked] -Nucleus removed by spinning. 1st PCR with poly-T and random primer. 2nd PCR nested and gene specific. - 15070753 [libraries] Library of DNAseI ends, to map hypersensitive sites. Substrraction with a HS-depleted tester. @@ -335,9 +332,6 @@ Alternative promoters which transcribe variants with different translational eff 14663149 [libraries] [tracked] 5' 20mers to map transcription start sites, and mesaure their usage. -11768647 [single cell] -Lysis in Dynabeads buffer, followed by first strand synthesis from poly-A. - 15342556 [genome] These blocks contain most validated and predicted transcription factor binding sites.