From: Charles Plessy Date: Fri, 22 Sep 2017 04:23:53 +0000 (+0900) Subject: Correction. X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=3bffb6d18441fe35a11e6935f11209a6bad25008;p=source.git Correction. --- diff --git a/biblio/12503310.mdw b/biblio/12503310.mdw deleted file mode 100644 index c28814e2..00000000 --- a/biblio/12503310.mdw +++ /dev/null @@ -1,10 +0,0 @@ -[[!meta title="Purification of histidine-tagged T4 RNA ligase from E. coli."]] -[[!tag enzyme ligase]] - -Biotechniques. 2002 Dec;33(6):1256-60 - -Wang QS, Unrau PJ. - -Purification of histidine-tagged T4 RNA ligase from E. coli. - -[[!pmid 12503310 desc="De-adenylation of T4 RNA ligase is required prior using adenylated substrates. Incubating with pyrophosphate during some purification steps does the job. Commercial T4 usually contains mostly de-adenylated enzyme. Mesuring reaction efficiency without ATP gives adenylation levels."]] diff --git a/biblio/12503310.mdwn b/biblio/12503310.mdwn new file mode 100644 index 00000000..c28814e2 --- /dev/null +++ b/biblio/12503310.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Purification of histidine-tagged T4 RNA ligase from E. coli."]] +[[!tag enzyme ligase]] + +Biotechniques. 2002 Dec;33(6):1256-60 + +Wang QS, Unrau PJ. + +Purification of histidine-tagged T4 RNA ligase from E. coli. + +[[!pmid 12503310 desc="De-adenylation of T4 RNA ligase is required prior using adenylated substrates. Incubating with pyrophosphate during some purification steps does the job. Commercial T4 usually contains mostly de-adenylated enzyme. Mesuring reaction efficiency without ATP gives adenylation levels."]]