From: Charles Plessy <plessy@riken.jp>
Date: Mon, 30 Oct 2017 07:06:10 +0000 (+0900)
Subject: Expand.
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=39d2d6598fd4b370957d43cc58d685ccc831098c;p=source%2F.git

Expand.
---

diff --git a/biblio/10518626.mdwn b/biblio/10518626.mdwn
index f2e1e169..f69b0d85 100644
--- a/biblio/10518626.mdwn
+++ b/biblio/10518626.mdwn
@@ -1,3 +1,10 @@
 [[!meta title="CapSelect: a highly sensitive method for 5' CAP-dependent enrichment of full-length cDNA in PCR-mediated analysis of mRNAs."]]
 [[!tag cap method enzyme manganese]]
+
+Nucleic Acids Res. 1999 Nov 1;27(21):e31.
+
+Schmidt WM, Mueller MW.
+
+CapSelect: a highly sensitive method for 5' CAP-dependent enrichment of full-length cDNA in PCR-mediated analysis of mRNAs.
+
 [[!pmid 10518626 desc="In presence of the 5' cap and Mn2+, the SuperScriptII enzyme adds 3-4 extra dC residues to the first strand cDNA."]]
diff --git a/tags/cap.mdwn b/tags/cap.mdwn
index 8c0c7c0c..b41aee63 100644
--- a/tags/cap.mdwn
+++ b/tags/cap.mdwn
@@ -1,4 +1,11 @@
 [[!meta title="pages tagged cap"]]
 
-[[!inline pages="tagged(cap)" actions="no" archive="yes"
-feedshow=10]]
+Methods for enriching capped RNAs.
+
+(work in progress)
+
+ - [[CapSelect|biblio/10518626.mdwn]]: Add a poly-A tail to the first-strand
+   cDNA, ligate a double-stranded adapter ending with a single-strand (T)TTTGGG
+   overhang, and prime second-strand synthesis.
+
+[[!inline pages="tagged(cap)" limit=0]]