From: Charles Plessy Date: Thu, 19 Apr 2018 02:36:28 +0000 (+0900) Subject: Earlier report. X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=3964baa9f9a6b051c0429f4df08811b2ae01bdab;p=source%2F.git Earlier report. --- diff --git a/biblio/2460825.mdwn b/biblio/2460825.mdwn index 1e7faae4..997b5cdb 100644 --- a/biblio/2460825.mdwn +++ b/biblio/2460825.mdwn @@ -1,6 +1,10 @@ [[!meta title="Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases."]] -[[!tag template_switching enzyme]] -[[!pmid 2460825 desc="‘we cannot exclude the formal possibility that some of these latter -events, particularly the addition of dCMP by AMV reverse transcriptase, involve the use of coding -information made available as a result of a transient misalignment of the primer/template -substrate.’"]] +[[!tag reverse_transcription enzyme polymerase]] + +Nucleic Acids Res. 1988 Oct 25;16(20):9677-86 + +Clark JM + +Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases. + +[[!pmid 2460825 desc="Terminal desoxynucleotidyl transferase activity of DNA polymerases, including AMV reverse transcriptase. Perference for adding As."]] diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn index 2f7c1952..696155c1 100644 --- a/tags/reverse_transcription.mdwn +++ b/tags/reverse_transcription.mdwn @@ -21,6 +21,9 @@ _(redaction in progress)_ ### Terminal desoxynucleotidyl transferase (TdT) activity +Like other DNA polymerases ([[Clark, 1988|biblio/2460825]]), reverse +transcriptases have a TdT activity. + - [[Chen & Patton, 2001|biblio/11252793]] reported a TdT activity for MMLV and AMV, with a preference for adding As. For MMLV, activity reduced abruptly between 45 and 50 °C. For AMV, it decreased constantly from 25 to 50 °C.