From: Charles Plessy Date: Tue, 1 Dec 2020 07:52:06 +0000 (+0900) Subject: re-read X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=380e431ad6be208ece0cad854b7cd2923289ed73;p=source.git re-read --- diff --git a/biblio/10471753.mdwn b/biblio/10471753.mdwn index 495da5a4..fc24caa9 100644 --- a/biblio/10471753.mdwn +++ b/biblio/10471753.mdwn @@ -7,6 +7,7 @@ Shagin DA, Lukyanov KA, Vagner LL, Matz MV. Regulation of average length of complex PCR product. -[[!pmid 10471753 desc="Amplify preferentially long PCR product with a single -primer. Shorter molecules have a higher probability of undergoing -inhibitory intramolecular interactions. (suppressive PCR)"]] +[[!pmid 10471753 desc="Suppression PCR amplification of a phage lambda digested +by HindII on which inverted terminal repeats were ligated. Suppression effect +is stronger when the PCR primer is shorter than the terminal repeats, and +when the PCR primer molarity is lower (tested in a 750–0 nM range)."]] diff --git a/tags/amplification.mdwn b/tags/amplification.mdwn index 531fee71..798fcbaf 100644 --- a/tags/amplification.mdwn +++ b/tags/amplification.mdwn @@ -5,6 +5,10 @@ _work in progress..._ ‘Suppression PCR’ was first published in English in [[Siebert and coll., 1995|biblio/7731798]]. Figure 1B shows a ‘panhandle’ structure. +Primer concentration (lower -> stronger suppression) and length ratio between +inverted tandem repeats and PCR primer (shorter PCR primer -> stronger +suppression) were investigated by Shagin and coll ([[1999|biblio/10471753]]). + Suppression PCR usually does not affect long (6~8 kbp) DNA molecules (mentionned in [[Lukyanov and coll., 2007|biblio/10.1007_978-1-4020-6040-3_2]]).