From: Charles Plessy Date: Mon, 5 Feb 2018 03:54:35 +0000 (+0900) Subject: Old X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=3787be1f25fcceeac0b8644e58ad5850648a3b00;p=source.git Old --- diff --git a/biblio/3799962.mdwn b/biblio/3799962.mdwn new file mode 100644 index 00000000..2bed881a --- /dev/null +++ b/biblio/3799962.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Ligation of single-stranded oligodeoxyribonucleotides by T4 RNA ligase."]] +[[!tag ligase]] + +Anal Biochem. 1986 Oct;158(1):171-8. + +Tessier DC, Brousseau R, Vernet T. + +Ligation of single-stranded oligodeoxyribonucleotides by T4 RNA ligase. + +[[!pmid 3799962 desc="ssDNA ligation. Optimal : Donor/acceptor 5:1; hexammine cobalt chloride 5.0 mM, PEG8000 25%; ATP 100µM. Inhibitors: ATP > 100 µM, MN 2+ > 5mM, NaCl > 20 mM."]] diff --git a/biblio/3978074.mdwn b/biblio/3978074.mdwn index b43d5086..718b8580 100644 --- a/biblio/3978074.mdwn +++ b/biblio/3978074.mdwn @@ -7,4 +7,4 @@ Biochemistry. 1985 Jan 15;24(2):267-73. Donor activation in the T4 RNA ligase reaction. -[[!pmid 3978074 desc="Adenylated RNA is a better substrate for T4 RNA ligase."]] +[[!pmid 3978074 desc="Adenylated RNA is a better substrate for T4 RNA ligase. Adenylation efficiency after 6h: C >> U ~ A > G."]] diff --git a/tags/ligase.mdwn b/tags/ligase.mdwn index 6fc507cc..afc1dd15 100644 --- a/tags/ligase.mdwn +++ b/tags/ligase.mdwn @@ -2,10 +2,18 @@ Work in progress. +Ligases are inhibited by high concentrations of ATP ([[Tessier, Brousserau & +Vernet, 1986|biblio/3799962]] and many others). + On T4 RNL1: - Using poly-A as substrate for circularisation, minimal length is 8, optimal is 10, and then efficiency decreases slowly ([[Kaufmann, Klein & Littauer, 1974|biblio/4609429]]). + - Adenylated RNA is a better substrate for T4 RNA ligase. + Adenylation efficiency after 6h: C >> U ~ A > G ([[McLaughlin et al., 1985|biblio/3978074]]). + - Also used for single-strand DNA ligation ([[Tessier, Brousserau & Vernet, 1986|biblio/3799962]]) + with PEG and hexamine cobalt chloride (HCC) as additives. + On T4 RNL2: