From: Charles Plessy Date: Thu, 4 Jan 2018 00:59:40 +0000 (+0900) Subject: Bonané X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=36502d78546ea9044e5eaa6b799bf698d774d010;p=source.git Bonané --- diff --git a/biblio/28911316.mdwn b/biblio/28911316.mdwn new file mode 100644 index 00000000..99d11249 --- /dev/null +++ b/biblio/28911316.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Optimizing T4 DNA polymerase conditions enhances the efficiency of one-step sequence- and ligation-independent cloning."]] +[[!tag library exonuclease method]] + +Biotechniques. 2017 Sep 1;63(3):125-130. doi:10.2144/000114588 + +Islam MN, Lee KW, Yim HS, Lee SH, Jung HC, Lee JH, Jeong JY. + +Optimizing T4 DNA polymerase conditions enhances the efficiency of one-step sequence- and ligation-independent cloning. + +[[!pmid 28911316 desc="Use of T4 DNA polymerase at 50 °C as an exonuclease for generating sinngle-strand overhangs."]]