From: Charles Plessy Date: Wed, 1 Nov 2017 08:42:17 +0000 (+0900) Subject: Old X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=33a89d735a895d8784271453a067cbc0e5a33fe7;p=source.git Old --- diff --git a/biblio/10471753.mdwn b/biblio/10471753.mdwn new file mode 100644 index 00000000..495da5a4 --- /dev/null +++ b/biblio/10471753.mdwn @@ -0,0 +1,12 @@ +[[!meta title="Regulation of average length of complex PCR product."]] +[[!tag PCR amplification]] + +Nucleic Acids Res. 1999 Sep 15;27(18):e23. + +Shagin DA, Lukyanov KA, Vagner LL, Matz MV. + +Regulation of average length of complex PCR product. + +[[!pmid 10471753 desc="Amplify preferentially long PCR product with a single +primer. Shorter molecules have a higher probability of undergoing +inhibitory intramolecular interactions. (suppressive PCR)"]] diff --git a/biblio/To_Do b/biblio/To_Do index c6266c00..51e46736 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -26,11 +26,6 @@ Review citing the 2 upper articles * Says that exponential (65+25 cycles) is more faithful than linear. * Limits [dNTP] to produce only 3' cDNA that are a few hundrer base pairs long. -10471753 -Amplify preferentially long PCR product with a single -primer. Shorter molecules have a higher probability of undergoing -inhibitory intramolecular interactions. - http://www.plosbiology.org/plosonline/?request=get-document&doi=10.1371%2Fjournal.pbio.0020178 ggcacgcga/cc => C-box for dro hairy. @@ -61,15 +56,6 @@ Single cell RT-PCR. 12711698 [amplification] [tracked] Uses T3N9 instead of T7dT during all the RT reactions. No 3' bias. Efficient on degraded RNA. Independant of polyadenylation (useful for non-polyA genes, like histones). -12560512 [amplification] [tracked] -«Semi-linear» Taq polymerase amplification used on the cDNA sample at the labelling step. - -14602935 [amplification] [tracked] -Performs SMART, then exponential PCR, then random-primed klenow labelling. - -12398200 [amplification] -Advocates a systematic round of RNA amplificatin, becaus it reduces interarray variability. - 15205470 [libraries] [tracked] T4 RNA ligase reaction efficiency improved by PEG and pre-adenylation. direct labelling method for big quantities: RNA fragmentation, then T4 RNA ligation of biotinylated oligos. @@ -580,9 +566,6 @@ In this changing network, most parameters except the clustering coefficient depe 15899964 [promoters] ChIP on chip focused on the preinitiation complexes of the ENCODE regions. -10471753 [amplification] -Single-primer PCR with a moderate suppressive effect can be used to regulate the product size. - 16407397 [enhancers] Idenification of conserved enhancers of shh hundreds of kp far of the coding sequence.