From: Charles Plessy <plessy@riken.jp>
Date: Fri, 20 Oct 2017 08:36:15 +0000 (+0900)
Subject: Old
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=3002a34caca2edd2a65cc899492d584f1c988b7b;p=source%2F.git

Old
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diff --git a/biblio/4000951.mdwn b/biblio/4000951.mdwn
new file mode 100644
index 00000000..7ea38871
--- /dev/null
+++ b/biblio/4000951.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Hexamine cobalt chloride promotes intermolecular ligation of blunt end DNA fragments by T4 DNA ligase."]]
+[[!tag ligation method]]
+
+Nucleic Acids Res. 1985 Mar 25;13(6):1997-2008.
+
+Rusche JR, Howard-Flanders P.
+
+Hexamine cobalt chloride promotes intermolecular ligation of blunt end DNA fragments by T4 DNA ligase.
+
+[[!pmid 4000951 desc="HCC (1 mM) promotes intermolecular ligation. This effect is countered by monovalent cations."]]
diff --git a/biblio/To_Do b/biblio/To_Do
index dffec3ea..fa189bd6 100644
--- a/biblio/To_Do
+++ b/biblio/To_Do
@@ -667,9 +667,6 @@ More inert than glycogen.
 12448877 [protocols]
 Ligating a DNA adaptor 3' to the FScDNA using T4RNA ligase.
 
-4000951 [protocols]
-HCC (1 mM) promotes intermolecular ligation. This effect is countered by monovalent cations.
-
 386284 [protocols]
 A low ATP concentration contributes to the attainment of high yields. ATP regenerating systems allow to work at sub-stoechiometric concentrations. Rnase A can be used as a SSBP. Spermine enhances yield.