From: Charles Plessy <plessy@riken.jp>
Date: Mon, 31 Jul 2017 08:29:37 +0000 (+0900)
Subject: Café.
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=2fb63b95a02c3365b8b56cd7d10451b46a422598;p=source%2F.git

Café.
---

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+[[!meta title="ChromEMT: Visualizing 3D chromatin structure and compaction in interphase and mitotic cells."]]
+[[!tag method chromatin microscopy]]
+
+Science. 2017 Jul 28;357(6349). pii: eaag0025. doi:10.1126/science.aag0025.
+
+Ou HD, Phan S, Deerinck TJ, Thor A, Ellisman MH, O'Shea CC.
+
+ChromEMT: Visualizing 3D chromatin structure and compaction in interphase and mitotic cells.
+
+[[!pmid 28751582 desc="The membrane-permeable anthraquinone dye DRAQ5 (646/697
+nm) was identified by a screen for its capacity to produce singlet oxygen,
+which polymerises diaminobenzidine (DAB), which binds the electron-dense osmium
+tetroxide (OsO4).  Use of this stain suggests that there are 30 nm fibers may
+be rare in vivo or an artefact of chromatin purification.  The authors suggest
+that the absence of higher-order fibers helps compaction, because disordered
+chromatin chains have shorter persistence length."]]