From: Charles Plessy <charles.plessy@oist.jp>
Date: Wed, 22 Mar 2023 08:35:16 +0000 (+0900)
Subject: PBS
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=2aadbe27b4bef6ddead89a805b2e0a09dd60dc99;p=source%2B%2B%2F.git

PBS
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+[[!meta title="Printing 2-dimentional droplet array for single-cell reverse transcription quantitative PCR assay with a microfluidic robot"]]
+[[!tag PCR]]
+
+Zhu Y, Zhang YX, Liu WW, Ma Y, Fang Q, Yao B.
+
+Sci Rep. 2015 Apr 1;5:9551. doi:10.1038/srep09551
+
+Printing 2-dimentional droplet array for single-cell reverse transcription quantitative PCR assay with a microfluidic robot
+
+[[!pmid 25828383 desc="For the amplification of a miRNA, the authors reported a
+98.03% efficiency in standard conditions.  It dropped to 90.94% with 0.5 mM
+49.99% PBS, 86.96% with 2 mM, 80.47% with 10 mM (1×), and 49.99% with 50 mM.
+The PCR was done in TaqMan universal PCR master mix."]]