From: Charles Date: Thu, 1 Oct 2020 04:43:30 +0000 (+0900) Subject: Café X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=289fa529a1ac0366108b33bdcf8a3d4f2d95cb2f;p=source%2F.git Café --- diff --git a/biblio/32879484.mdwn b/biblio/32879484.mdwn new file mode 100644 index 00000000..a49aacb0 --- /dev/null +++ b/biblio/32879484.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Biosynthesis of medicinal tropane alkaloids in yeast."]] +[[!tag yeast synthetic]] + +Srinivasan P, Smolke CD. + +Nature. 2020 Sep;585(7826):614-619. doi:10.1038/s41586-020-2650-9 + +Biosynthesis of medicinal tropane alkaloids in yeast. + +[[!pmid 32879484 desc="“Our final strain comprises 34 chromosomal modifications (26 genes, 8 gene disruptions), resulting in an integrated whole-cell system that expresses enzymes and transporters in diverse sub-cellular locations (cytosol, mitochondria, peroxisome, vacuole, ER and vacuolar membranes)” Screened a plant RNA-seq to discover one of the enzymes. Protein sequence needed modifications for proper trans-compartment transport and maturation in the yeast cells."]]