From: Charles Plessy Date: Tue, 31 Aug 2021 19:41:24 +0000 (+0900) Subject: matin X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=2849b1cde22b9042a113cf6f6a2d2db5160dd437;p=source.git matin --- diff --git a/biblio/34400545.mdwn b/biblio/34400545.mdwn new file mode 100644 index 00000000..46552aab --- /dev/null +++ b/biblio/34400545.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Ultrarapid detection of SARS-CoV-2 RNA using a reverse transcription-free exponential amplification reaction, RTF-EXPAR."]] +[[!tag amplification]] + +Carter JG, Orueta Iturbe L, Duprey JHA, Carter IR, Southern CD, Rana M, Whalley CM, Bosworth A, Beggs AD, Hicks MR, Tucker JHR, Dafforn TR. + +Proc Natl Acad Sci U S A. 2021 Aug 31;118(35):e2100347118. doi:10.1073/pnas.2100347118 + +Ultrarapid detection of SARS-CoV-2 RNA using a reverse transcription-free exponential amplification reaction, RTF-EXPAR. + +[[!pmid 34400545 desc="“Binder DNA X anneals to viral RNA; the DNA strand of the DNA:RNA heteroduplex is cut by the restriction endonuclease BstNI, which acts as a nicking enzyme by cutting the DNA strand only; the released DNA strand is Trigger X, which is then amplified by EXPAR.”"]]