From: Charles Plessy Date: Tue, 24 Oct 2017 01:48:44 +0000 (+0900) Subject: Old X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=22e86bc7a10c6abbcfb8458090f765657016a4bb;p=source.git Old --- diff --git a/biblio/386284.mdwn b/biblio/386284.mdwn new file mode 100644 index 00000000..6a78a5a7 --- /dev/null +++ b/biblio/386284.mdwn @@ -0,0 +1,10 @@ +[[!meta title="The synthesis of oligodeoxyribonucleotides using RNA ligase."]] +[[!tag ligase enzyme]] + +Nucleic Acids Res. 1979 Sep 25;7(2):453-64. + +Hinton DM, Gumport RI. + +The synthesis of oligodeoxyribonucleotides using RNA ligase. + +[[!pmid 386284 desc="A low ATP concentration contributes to the attainment of high yields. ATP regenerating systems allow to work at sub-stoechiometric concentrations. Rnase A can be used as a SSBP. Spermine enhances yield."]] diff --git a/biblio/To_Do b/biblio/To_Do index 8c864e31..4a421b52 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -661,9 +661,6 @@ The PPi produced during the PCR can be removed with the pyrosequencing enzymes t 12448877 [protocols] Ligating a DNA adaptor 3' to the FScDNA using T4RNA ligase. -386284 [protocols] -A low ATP concentration contributes to the attainment of high yields. ATP regenerating systems allow to work at sub-stoechiometric concentrations. Rnase A can be used as a SSBP. Spermine enhances yield. - 9380524 [protocols] Concentrations as high as 1 M or 2.5 M can strongly improve the yield.