From: Charles Plessy Date: Tue, 30 May 2017 04:32:57 +0000 (+0900) Subject: Au bureau. X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=1a223e2c37394cb8a61310155bc506ec4094872d;p=source%2F.git Au bureau. --- diff --git a/biblio/17332513.mdwn b/biblio/17332513.mdwn new file mode 100644 index 00000000..9f7db377 --- /dev/null +++ b/biblio/17332513.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24."]] +[[!tag DNAse_I method]] + +Stem Cells. 2007 Jun;25(6):1560-70. Epub 2007 Mar 1. + +Panchision DM, Chen HL, Pistollato F, Papini D, Ni HT, Hawley TS. + +Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24. + +[[!pmid 17332513 desc='"On two occasions with papain and three occasions with TrypLE, we saw free-DNA aggregation even in the presence of DNase I, indicating substantial cell lysis. We could not definitively determine the cause of this variability. Two additional papain and one TrypLE sample could not be properly assayed by hemocytometer because of DNA aggregation and so were discarded. Accutase and Liberase-1 never generated free DNA aggregates."']] diff --git a/biblio/28526029.mdwn b/biblio/28526029.mdwn index 08b22aef..99abfae7 100644 --- a/biblio/28526029.mdwn +++ b/biblio/28526029.mdwn @@ -1,5 +1,5 @@ [[!meta title="Cell fixation and preservation for droplet-based single-cell transcriptomics."]] -[[!tag methanol fixation method single_cell]] +[[!tag methanol fixation method single_cell DNAse_I]] Alles J, Karaiskos N, Praktiknjo SD, Grosswendt S, Wahle P, Ruffault PL, Ayoub S, Schreyer L, Boltengagen A, Birchmeier C, Zinzen R, Kocks C, Rajewsky N.