From: Charles <charles.plessy@oist.jp>
Date: Wed, 2 Sep 2020 06:05:00 +0000 (+0900)
Subject: Café
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=1619e61c07bd712c09bce3cd765c9ebc795710a9;p=source.git

Café
---

diff --git a/biblio/1465136.mdwn b/biblio/1465136.mdwn
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+[[!meta title="Spliced leader RNAs from lower eukaryotes are trans-spliced in mammalian cells."]]
+[[!tag trans-splicing]]
+
+Bruzik JP, Maniatis T.
+
+Nature. 1992 Dec 17;360(6405):692-5. doi:10.1038/360692a0
+
+Spliced leader RNAs from lower eukaryotes are trans-spliced in mammalian cells.
+
+[[!pmid 1465136 desc="Trans-splicing in vivo in COS cells and in vitro in HeLa cell extracts, using splice leaders and actin genes from C. elegans and Leptomonas colosoma, and also using an adenoviral RNA as acceptor."]]
diff --git a/tags/trans-splicing.mdwn b/tags/trans-splicing.mdwn
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 [[!meta title="pages tagged trans-splicing"]]
 
+Trans-spplicing of a splice leader from a nematode and a trypanosome was shown
+to be possible in COS cells and HeLa cell extracts by [[Bruzik and Maniatis, 1992|biblio/1465136]].
+
 Trans-splicing was discovered in tunicates by [[Vandenberghe, Meedel and Hastings, 2001|biblio/11159910]].
 
 The splice leader is 16-nt in C. intestinalis ([[Satou et al, 2006|biblio/16822859]]) and 40-nt in O. dioica ([[Ganot et al., 2004|biblio/15314184]]).