From: Charles Plessy Date: Mon, 30 Oct 2017 07:55:55 +0000 (+0900) Subject: Old X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=15e48e5555a4deb1dfdba35989781ee8b495e547;p=source%2F.git Old --- diff --git a/biblio/14704363.mdwn b/biblio/14704363.mdwn new file mode 100644 index 00000000..6816e33e --- /dev/null +++ b/biblio/14704363.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Improved full-length cDNA production based on RNA tagging by T4 DNA ligase."]] +[[!tag cap method]] + +Nucleic Acids Res. 2004 Jan 2;32(1):e6 doi:10.1093/nar/gng158 + +Clepet C, Le Clainche I, Caboche M. + +Improved full-length cDNA production based on RNA tagging by T4 DNA ligase. + +[[!pmid 14704363 desc="T4 DNA ligase used to ligate a double stranded cap-tag."]] diff --git a/biblio/To_Do b/biblio/To_Do index fbc74317..8db5444d 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -80,9 +80,6 @@ Oligo-capping to build 5' ou FL cDNA libraries. 14606961 [libraries] The nuclease activity of T7 RNA pol could also show some transcript specificity. -14704353 [libraries] [tracked] -T4 DNA ligase used to ligate a double stranded cap-tag. - 3799962 [enzymes] Optimal : Donor/acceptor 5:1; hexammine cobalt chloride 5.0 mM, PEG8000 25%; ATP 100µM. Inhibitors: ATP > 100 µM, MN 2+ > 5mM, NaCl > 20 mM. diff --git a/tags/cap.mdwn b/tags/cap.mdwn index b21b1281..cc9b7317 100644 --- a/tags/cap.mdwn +++ b/tags/cap.mdwn @@ -4,8 +4,11 @@ Methods for enriching capped RNAs. (work in progress) - - [[CapSelect|biblio/10518626]]: Add a poly-A tail to the first-strand + - CapSelect [[Schmidt et al., 1999|biblio/10518626]]: Add a poly-A tail to the first-strand cDNA, ligate a double-stranded adapter ending with a single-strand (T)TTTGGG overhang, and prime second-strand synthesis. + - [[Cleptet et al., 2004|biblio/14704363]] used oligo-capping, but used T4 DNA ligase and a double-stranded + adapter with NNNNNN overhang instead of T4 RNA ligase and a single-stranded linker. + [[!inline pages="tagged(cap)" limit=0]]