From: Charles Plessy <https://launchpad.net/~plessy>
Date: Wed, 4 Jun 2014 09:15:05 +0000 (+0900)
Subject: C'était l'heure de la soupe.
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=14da08380b29a046e91ff96585e2708108d299c3;p=source.git

C'était l'heure de la soupe.
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+[[!meta title="Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green I depends on cDNA synthesis conditions."]]
+[[!tag DTT reverse_transcription qPCR]]
+
+Lekanne Deprez RH, Fijnvandraat AC, Ruijter JM, Moorman AF.
+
+Anal Biochem. 2002 Aug 1;307(1):63-9.
+
+Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green I depends on cDNA synthesis conditions.
+
+[[!pmid 12137780 desc="DTT interferes with fluorescence measurements in qPCR.  RT without DTT is efficient.  Random-primerd replicates are less reproducible than oligo-dT or gene-specific-primed ones."]]