From: Charles Plessy <plessy@riken.jp>
Date: Tue, 15 May 2018 00:31:06 +0000 (+0900)
Subject: slack
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=12cd74d3921bd0eaf8a9592cd656d1ff90f6845a;p=source%2F.git

slack
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diff --git a/biblio/26152304.mdwn b/biblio/26152304.mdwn
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+[[!meta title="A new method to prevent carry-over contaminations in two-step PCR NGS library preparations."]]
+[[!tag library amplification method]]
+
+Seitz V, Schaper S, Dröge A, Lenze D, Hummel M, Hennig S.
+
+Nucleic Acids Res. 2015 Nov 16;43(20):e135. doi:10.1093/nar/gkv694
+
+A new method to prevent carry-over contaminations in two-step PCR NGS library preparations.
+
+[[!pmid 26152304 desc="Proposes to introduce indexes during the cDNA PCR, and to partially match them during Library PCR, so that 1) the PCR is more stringent and 2) contaminations can be detected by sequence analysis."]]